近日,Frontiers in Plant Science 在線發表中國農大王建華和中國農科院劉允軍團隊題為“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研究論文,系統解析了半胱氨酸提高農桿菌介導的玉米幼胚轉化效率的機理。 最近幾年,玉米的遺傳轉化效率不斷提高,其中抗氧化劑半胱氨酸可明顯提高玉米幼胚的農桿菌轉化效率,但其機理尚不明確,在本研究中作者證明通過在共培養培養基中添加半胱氨酸可以明顯HiII和Z31兩個品種的農桿菌轉化效率,半胱氨酸處理后的幼胚活性氧的含量要高于對照組,通過轉錄組測序進一步研究證明,半胱氨酸處理后939個基因表達上調,這些基因主要參與氧化還原反應過程,另外有549個基因表達下調,主要參與細胞壁和細胞膜代謝過程。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...
The free-living flatworm, Macrostomum lignano has an impressive regenerative capacity. Following injury, it can regenerate almost an entirely new organism because of the presence of an abundant somatic stem cell population, the neoblasts. This set of unique properties makes many flatworms attractive...
An overview of our assembly strategy is shown in Figure 1. We started with a SOAPdenovo- based de novo assembly of Illumina sequence data from human HapMap sample NA12878, which had a contig N50 of 11.1 kb and a scaffold N50 of 590 kb after filtering for scaffolds that were at least 3 kb in length (...
Transposable elements and expression of retrotransposons Long terminal repeat (LTR) retrotransposons were identified using structural criteria10,11. About 44% of the assembly was accounted for by TEs (Supplementary Table 8 ). LTR retrotransposons were the most abundant of these elements, repres...
Winter dormancy is a well known mechanism adopted by temperate plants, to mitigate the chilling temperature of winters. However, acquisition of sufficient chilling during winter dor-mancy ensures the normal phenological traits in subsequent growing period. Thus, low tem-perature appears to play cruc...
Whole genome protein families were classified by InterProScan analysis (Jones et al., 2014). The peptidase families were identified by BLASTP searching against MEROPS peptidase database release 9.12 with a cuto? e-value of 1e-20 (Rawlings et al., 2014). The transporters were classified on the b...
近日,Frontiers in Plant Science 在線發表中國農大王建華和中國農科院劉允軍團隊題為“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研究論文,系統解析了半胱氨酸提高農桿菌介導的玉米幼胚轉化效率的機理。 最近幾年,玉米的遺傳轉化效率不斷提高,其中抗氧化劑半胱氨酸可明顯提高玉米幼胚的農桿菌轉化效率,但其機理尚不明確,在本研究中作者證明通過在共培養培養基中添加半胱氨酸可以明顯HiII和Z31兩個品種的農桿菌轉化效率,半胱氨酸處理后的幼胚活性氧的含量要高于對照組,通過轉錄組測序進一步研究證明,半胱氨酸處理后939個基因表達上調,這些基因主要參與氧化還原反應過程,另外有549個基因表達下調,主要參與細胞壁和細胞膜代謝過程。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent...